Logo RUB
  • Institute
    • ICAMS
      • Mission
      • Structure
      • Members
      • Fellows
    • Departments & Research Groups
      • Atomistic Modelling and Simulation
      • Scale-Bridging Thermodynamic and Kinetic Simulation
      • Micromechanical and Macroscopic Modelling
      • Artificial Intelligence for Integrated Material Science
      • Computational Design of Functional Interfaces
      • Scale-Bridging Simulation of Functional Composites
      • Materials Informatics and Data Science
      • High-Performance Computing in Materials Science
    • Central Services
      • Coordination Office
      • IT
  • Research
    • Overview
    • Publications
    • Software and Data
    • Collaborative research
    • Research networks
    • Young enterprises
  • Teaching
    • Overview
    • Materialwissenschaft B.Sc.
    • Materials Science and Simulation M.Sc.
    • ICAMS Graduate School
    • Student Projects
  • News & Events
    • Overview
    • News
    • Seminars and Workshops
    • Conferences
  • Services
    • Overview
    • Contact
    • Open positions
    • Travel information
 
ICAMS
ICAMS
MENÜ
  • RUB-STARTSEITE
  • Institute
    • ICAMS
    • Departments & Research Groups
    • Central Services
  • Research
    • Overview
    • Publications
    • Software and Data
    • Collaborative research
    • Research networks
    • Young enterprises
  • Teaching
    • Overview
    • Materialwissenschaft B.Sc.
    • Materials Science and Simulation M.Sc.
    • ICAMS Graduate School
    • Student Projects
  • News & Events
    • Overview
    • News
    • Seminars and Workshops
    • Conferences
  • Services
    • Overview
    • Contact
    • Open positions
    • Travel information

Just another WordPress site - Ruhr-Universität Bochum

Microwave-assisted synthesis of thiophene fluorophores, labeling and multilabeling of monoclonal antibodies and long lasting staining of fixed cells

M. Zambianchi, F. Di Maria, A. Cazzato, G. Gigli, M. Piacenza, F. Della Sala, G. Barbarella

Journal of the American Chemical Society, 131, 10892-10900, (2009)

DOI: 10.1021/ja902416s

Download: BibTEX

We report the expedient microwave-assisted synthesis of thiophene based 4-sulfo-2,3,5,6,- tetrafluorophenyl esters whose molecular structure was engineered to achieve blue to red bright fluorescence. The reactivity toward monoclonal antibodies of the newly synthesized fluorophores was analyzed in comparison with that of the corresponding N-succinimidyl esters. Single-fluorophore and multiplefluorophore labeled antibodies were easily prepared with both types of esters. Multiple-fluorophore labeling with blue and orange emitting fluorophores resulted in white fluorescent antibodies. Thiophene based fluorophores displayed unprecedented fluorescence stability in immunostaining experiments. First-principles TD-DFT theoretical calculations helped us to interpret the behavior of fluorescence emission in different environments.

back
{"type":"article", "name":"m.zambianchi20098", "author":"M. Zambianchi and F. Di Maria and A. Cazzato and G. Gigli and M. Piacenza and F. Della Sala and G. Barbarella", "title":"Microwaveassisted synthesis of thiophene fluorophores, labeling and multilabeling of monoclonal antibodies and long lasting staining of fixed cells", "journal":"Journal of the American Chemical Society", "volume":"131", "OPTnumber":"31", "OPTmonth":"8", "year":"2009", "OPTpages":"10892-10900", "OPTnote":"", "OPTkey":"Semiconductor quantum dots; N-succinimidyl esters; fluorescent proteins; conjugated polymers; energy-transfer; dyes; bright; probes; molecule;density", "DOI":"10.1021/ja902416s"}
Logo RUB
  • Open positions
  • Travel information
  • Imprint
  • Privacy Policy
  • Sitemap
Ruhr-Universität Bochum
Universitätsstraße 150
44801 Bochum

  • Open positions
  • Travel information
  • Imprint
  • Privacy Policy
  • Sitemap
Seitenanfang Kontrast N